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Tag: ASCP
Common ANA (ImmunoFlourescence) Test Patterns
These are the common patterns for the ANA/IF test.
Adrenal gland
Anatomy
- medulla == inner layer == catecholamines
- cortex == outer layer divides into 1. zona glomerulosa == mineralocorticoids 2. zona fasciculata == glucorticoid 3. inner zona reticularis == androgens
Pheochromocytoma = catecholamine producing == VMA,metanephrine,
test metanephrine levels plasma and urine
Source:
Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 edition
p.342
Anemia Classification
IDA ( Iron Deficiency Anemia )
- 1 ml of RBC == 1 mg of Fe
- Ferritin ↓– storage Iron along with hemosiderin . This is a complex of ferric salt and apoferritin ,protein . differentiates it from ACD,thallasemia,Sideroblastic
- transferrin – transport protein = TIBC ↑
- characterized by hypochromic, microcytic anemia
- MCV ↓ , HCT & HgB ↓
Megaloblastic Anemia
- Macrocytic
- MCV ↑
- B12 and Folic deficiency
- Pernicious Anemia – failure of the gastric mucosa to secrete intrinsic factor
Anemia of Chronic Diseases(ACD)
- Normocytic,normochromic although 20 – 50 % would be microcytic hypochromic
- SI ↓, TIBC normal/↓ ,Ferritin ↑
Sideroblastic Anemia
- Hypochromic, often microcytic mixed with normochromic = dimorphic
- SI ↑, TIBC ↓, % SAT ↑↑
- “ring sideroblasts” in marrow
Blood Loss Anemia
- Acute – normocytic, normochromic
- Chronic – microcytic then hypochromic
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Macrocytic Anemia(Increased MCV)
normochromic base on the film and by the MCHC
- Megaloblastic
- Non-Megaloblastic
Microcytic and Hypochromic Anemias(Decreased MCV and MCH)
MCHC = normal
- IDA
- ACD
- Thallasemia
- Sideroblastic
Normocytic and Normochromic Anemia(Normal MCV)
check Reticulocyte production index and Bone marrow
DAT
hemolytic anemias
SOURCE:
Source:
Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 edition
Chapter 31
RBC Indeces
Here are the formulas for RBC indeces:
- Mean Cell Volume(MCV) MCV=Hct x 10 / RBC in millions/ mm3 Normal=82-92 femtoliters or base on clin diag 80 – 96 fL or µm3
- Mean Cell Hemoglobin(MCH) MCH=gm Hgb /dL x 10 / RBC in millions/mm3 Normal=27-32 micromicrograms | clin diag 27.5 – 33.2 pg
- Mean Cell Hemoglobin Concentration(MCHC) MCHC=gm Hgb/dL x 100 /Hct % Normal=32-36 % | clin diag 33.4 – 35.5
Typical Red Cell count
Men 4.5 – 5.9 x 10 6 /µL
Female 4.5 – 5.1
Hematocrit
Male 41.5 – 50.4 %
Female 35.9 – 44.6
Hemoglobin
Male 14 – 17.5 g/dL
Female 12.3 – 15.3
Source:
Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 edition
p.460,1414
